Fusarium Laboratory Workshop
This workshop is taught by international Fusarium experts. Participants are introduced to standard morphological, genetic and molecular biological techniques used to identify and characterize strains of Fusarium .
Participants will learn to use morphological characters to identify the most common Fusarium species, how to make tests for vegetative compatibility groups (VCGs) and cross-fertility, and how to extract, PCR amplify DNA, and analyze DNA sequences. More than half of the time will be spent in the laboratory working with standard strains. Students may bring some of their own strains (please contact Dr. Leslie to arrange for proper USDA permits).
UPCOMING WORKSHOP 21-26 June 2026
Laboratory Strain Identification
Molecular Identification
Species Concepts
Mating Types and Crosses
VCG Analysis
Strain Preservation
Mycotoxins
Erick DeWolf
Steve Ensley
David Geiser
Antonio Logrieco
Gary Munkvold
Brett Summerell
Christopher Toomajian
Anne van Diepeningen
Jin-Rong Xu
John F. Leslie
Kansas State University
SUNDAY, JUNE 21ST
Check into hotel/dormitory, workshop materials available at the front desk when you check in.
Evening 6:00 - 8:30 pm
- Social hour and dinner - Konza Prairie Biological Station
MONDAY, JUNE 22ND
Morning 8:00 am - Noon
- Welcome from the university administration
- Introduction to morphology, phylogenetics and taxonomy
LUNCH (on your own)
Afternoon 1:00 - 5:00 pm
- Species concepts
- Fusarium sambucinum species complex - Part 1
- Single spore isolation
- Soil dilution isolate recovery
- Plant sample isolate recovery
TUESDAY, JUNE 23RD
Morning 8:00 am - Noon
- Fusarium head blight modeling
- Trichothecenes and zearalenone
- Temperate region Fusarium species and diseases
- Vegetative compatibility groups and tests
LUNCH (on your own)
Afternoon 1:00 - 5:15 pm
- F. oxysporum and F. solani species complexes and basal lineages
- Single-spore laboratory follow-up
- Strain preservation and conservation
Morning 8:00 am - Noon
- Fusarium fujikuroi species complex - lecture
- Fumonisins and other mycotoxins
- Fusarium plant pathogenicity mechanisms
- Mycotoxin detection - Principles and practice
LUNCH (on your own)
Afternoon 1:00 - 5:15 pm
- Population genetics
- DNA sequence laboratory
- Fusarium fujikuroi species complex - laboratory
THURSDAY, JUNE 25TH
Morning 8:00 am - Noon
- Fusarium incarnatum species complex
- Tropical region Fusarium species and diseases
- Mating type - lecture
Afternoon 1:00 - 5:00 pm
- Fusarium sambucinum species complex - II
- Fusarium tricinctum species complex
- Fusarium genetics and genomics
- Mating type - laboratory
- Morphology quiz
- Workshop pool party (Dr. Leslie’s house)
FRIDAY, JUNE 26TH
Morning 8:30 - 11:30 am
- Mycotoxin detection and analysis
- Fusarium in human and domesticated animals
- Closing thoughts
- Distribution of certificates
- Workshop evaluation
Registration requires a nonrefundable $100 deposit to reserve space. Registration is not complete until the fees are paid. Full payment is due May 1. Registration after May 22 is on a space-available basis only. Enrollment is limited. Virtual attendance is not available due to lab work.
Send registration questions to fusariumworkshop@ksu.edu.
Workshop Contact Information
Email: fusariumworkshop@ksu.edu
Phone: +1 (785) 532-1363
Erick DeWolf
Professor in the Department of Plant Pathology at Kansas State University. His research focuses on modeling and plant disease forecasting systems, with recent emphasis on Fusarium head blight.
Steve Ensley
DVM and Ph.D. in Toxicology; Director of the KSU Veterinary Diagnostic Lab. Routinely quantifies mycotoxin contamination and clinical mycotoxicosis cases in food and companion animals.
David Geiser
Professor in the Department of Plant Pathology and Director of the Fusarium Research Center at Pennsylvania State University. Has extensive experience in the molecular evolution of fungi and fungal population genetics.
John F. Leslie
University Distinguished Professor in the Department of Plant Pathology at Kansas State University. Has over 40 years of experience with Fusarium genetics and population analysis.
Antonio Logrieco
Emeritus Director of the Institute for the Science of Food Production, National Council of Research (CNR‑ISPA), Italy. Specializes in epidemiology, taxonomy, and molecular/chemical characterization of toxigenic Fusarium species.
Gary Munkvold
Chris Toomajian
Associate Professor in the Department of Plant Pathology at Kansas State University. His interests include fungal genomics, population genetics, and speciation, with expertise in the molecular signature of natural selection.
Brett Summerell
Director of Science and Conservation at the Royal Botanic Garden Sydney (Australia). Has over 30 years of experience in the taxonomy, identification, and description of Fusarium species and the plant diseases they cause. Co‑author of two lab manuals on Fusarium identification.
Anne Van Diepeningen
A fungal geneticist in the Biointeractions and Plant Health unit of Wageningen University and Research. Previously worked at the Westerdijk Fungal Biodiversity Institute studying Fusarium infections in humans and animals; currently focuses on plant pathogens and mycotoxigenic species.
Jin‑Rong Xu
Professor in Botany and Plant Pathology at Purdue University. Has 25 years of experience with classic, molecular, and genomic studies of F. graminearum and F. verticillioides, particularly the signal transduction pathways that regulate pathogenesis and development.
This workshop will be taught by international Fusarium experts. Participants will be introduced to standard morphological, genetic, and molecular biological techniques used to identify and characterize strains of Fusarium.
Participants will learn:
- How to use morphological characters to identify common Fusarium species
- How to perform vegetative compatibility group (VCG) tests and cross‑fertility tests
- How to extract DNA, perform PCR amplification, and analyze DNA sequences
More than half of the workshop time will be spent in the laboratory working with standard strains. Students may send their own strains (contact John Leslie to arrange proper USDA permits).
(USDA permit required for U.S. residents; send a photocopy of the permit to John Leslie prior to the workshop.)
Strain set costs are in addition to workshop registration fees.
Optional on‑campus housing, including all meals, will be available during the workshop (June 21–26, ending with lunch) for $400. Additional nights may be added for $85/day.
A block of rooms is also available at three hotels adjacent to campus:
- Bluemont Hotel
- Marriott Courtyard
- Holiday Inn at Campus
Participants should contact hotels directly for rates and reservations.
Participants are responsible for all transportation costs, including visas, and for their accommodations and meals (except those noted) while in Manhattan.
The $2,900 registration fee includes:
- Workshop materials and handouts
- A copy of the Fusarium Laboratory Manual
- Welcome and workshop dinners
- Lab supplies
- Certificate of participation
- Refreshment breaks each day
Limited financial assistance is available.
Contact John Leslie at +1 (785) 532‑1363 or jfl@ksu.edu.
Payment must be in USD. All bank fees, wire charges, and service charges are the responsibility of the registrant.
Early notification - by May 22 - is requested to ensure accommodations can be arranged.
For workshop or course information, contact John Leslie at +1 (785) 532‑1363 or jfl@ksu.edu.
If you require a visa or a letter of invitation, contact John Leslie. Invitation letters will be emailed; original letters will not be sent to embassies, and no additional visa assistance can be provided.
If you must cancel, please do so as soon as possible. Substitutions are accepted.
Registration fees, less a $100 deposit, will be refunded if cancellation is received by 5 p.m. CDT on May 22.
After that date, no refunds will be issued. Fees will not be canceled for registered participants who do not attend and did not notify the workshop coordinator or John Leslie before the deadline.
Kansas State University and the workshop coordinator may cancel or postpone any course or activity of the workshop due to insufficient enrollment or unforeseen circumstances. If canceled or postponed, registration fees will be refunded, but the University cannot reimburse travel or other expenses.
Refunds are not issued if the program is held but you cannot attend due to travel delays, weather, or circumstances beyond the control of Kansas State University.
Inquiries and complaints:
Institutional Equity (equity@ksu.edu)
Kansas State University
220A Kedzie Hall
Manhattan, KS 66506‑4801
Phone: 1‑785‑532‑6220
TTY: 1‑785‑532‑4807
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